Fluorescence imaging and image analysis of actin dynamics in living cell
نویسندگان
چکیده
Dynamic assembly of actin cytoskeleton drives multiple intracellular biological processes in eukaryotic cells. Different types of actin networks are generated upon cellular requirement in a temporaland spatial-specific manner. Living cell imaging of dynamic behaviors of actin filaments provides the mechanistic insights for the actin polymerization, which enhances our understanding of actin assembly per se and the actin powered biological processes. High quality living cell images enable quantitative measurement of actin dynamics that make great contributions to our knowledge of intracellular regulation of actin assembly. In this chapter, we will demonstrate the approaches for fluorescent living cell imaging and image analysis for studying actin filament assembly in budding yeast. Advanced wild-field microscope, imaging acquisition and deconvolution approaches will be used to quantitatively determine the lifetime of actin patches and the dynamics of actin cables, which support clathrin-mediated endocytosis and membrane transport, respectively.
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تاریخ انتشار 2017